GATE 2017-2018 :: GATE Biotechnology
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Match the entries in Group I with the methods of sterilization in Group II.Group I Group IIP. Serum 1. AutoclaveQ. Luria broth 2. Membrane filtrationR. Polypropylene tubes 3. UV irradiationS. Biological safety cabinets 4. Gamma irradiation5. Dry heat
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Match the high energy compounds in Group I with the biosynthetic pathways for the molecules in Group II.Group I Group IIP. GTP 1. Fatty acidQ. UTP 2. PhospholipidR. CTP 3. ProteinS. Acyl coenzyme A 4. Peptidoglycan
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Match the vitamins in Group I with the processes/reactions in Group II.Group I Group IIP. Pantothenic acid 1 . Electron transportQ. Vitamin B2 2. Transfer of 1-C unitsR. Vitamin B6 3. DecarboxylationS. Folic acid 4 . Fatty acid metabolism5. Hydrolysis
- Consider the data set 14, 18, 14, 14, 10, 29, 33, 31, 25. If you add 20 to each of the values, then
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An enzymatic reaction is described by the following rate expression. v = (vms)/(km + s + s2/ks)Which one of the following curves represents this expression?
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A bacterial culture (200 ïl containing 1.8 * 109 cells) was treated with an antibiotic Z (50 μg per ml) for 4 h at 37ËšC. After this treatment, the culture was divided into two equal aliquots. Set A: 100 μl was plated on Luria agar.Set B: 100 μl was centrifuged, the cell pellet washed and plated on Luria agar.After incubating these two plates for 24 h at 37ËšC, Set A plate showed no colonies, whereas the Set B plate showed 0.9 * 109 cells. This experiment showed that the antibiotic Z is
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In a muscle, the extracellular and intracellular concentrations of Na+ are 150 mM and 12 mM, and those of K+ are 2.7 mM and 140 mM, respectively. Assume that the temperature is 25oC and that the membrane potential is -60 mV, with the interior more negatively charged than the exterior. (R = 8.314 J mol-1 K-1; F = 96.45 kJ mol-1 V-1) The free energy change for the transport of three Na+ out of the cell is
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In a muscle, the extracellular and intracellular concentrations of Na+ are 150 mM and 12 mM, and those of K+ are 2.7 mM and 140 mM, respectively. Assume that the temperature is 25oC and that the membrane potential is -60 mV, with the interior more negatively charged than the exterior. (R = 8.314 J mol-1 K-1; F = 96.45 kJ mol-1 V-1) The free energy change for the transport of two K+ into the cell is
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The purification data for an enzyme is given below:
The fold purification for each step is -
The purification data for an enzyme is given below:
The yield (%) for each step is
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