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GATE 2017-2018 :: GATE Biotechnology

  1. A protein is phosphorylated at a serine residue. A phosphomimic mutant of the protein can be generated by substituting that serine with
  2. A.
    glycine
    B.
    alanine
    C.
    aspartate
    D.
    threonine

  3. A truncated polypeptide is synthesized due to a nonsense mutation. Where would you introduce another mutation to obtain a full-length polypeptide?
  4. A.
    Ribosomal protein gene
    B.
    Transfer RNA gene
    C.
    DNA repair gene
    D.
    Ribosomal RNA gene

  5. Protein-DNA interactions in vivo can be studied by
  6. A.
    gel shift assay
    B.
    Southern hybridization
    C.
    chromatin immunoprecipitation assay
    D.
    fluorescence in situ hybridization assay

  7. The direction of shell coiling in the snail Limnaea peregra is a classic example of
  8. A.
    chromosomal inheritance
    B.
    extra-chromosomal inheritance
    C.
    chromosomal translocation
    D.
    homologous recombination

  9. During photorespiration under low CO2 and high O2 levels, O2 reacts with ribulose 1,5- bisphosphate to yield
  10. A.
    one molecule each of 3-phosphoglycerate and 2-phosphoglycolate
    B.
    two molecules of 3-phosphoglycerate
    C.
    two molecules of 2-phosphoglycolate
    D.
    one molecule each of 3-phosphoglycerate and glyoxylate

  11. Which one of the following is NOT a protoplast fusion inducing agent?
  12. A.
    Inactivated Sendai virus
    B.
    Ca2+ at alkaline pH
    C.
    Polyethylene glycol
    D.
    Colchicine

  13. The activity of an enzyme is expressed in International Units (IU). However, the S.I. unit for enzyme activity is Katal. One Katal is
  14. A.
    1.66 * 104 IU
    B.
    60 IU
    C.
    6 * 107 IU
    D.
    106 IU

  15. Identify the statement that is NOT applicable to an enzyme catalyzed reaction.
  16. A.
    Enzyme catalysis involves propinquity effects
    B.
    The binding of substrate to the active site causes a strain in the substrate
    C.
    Enzymes do not accelerate the rate of reverse reaction
    D.
    Enzyme catalysis involves acid-base chemistry

  17. An example of a derived protein structure database is
  18. A.
    Pfam
    B.
    SCOP
    C.
    GEO
    D.
    Prosite

  19. An example of a program for constructing a phylogenetic tree is
  20. A.
    phylip
    B.
    phrap
    C.
    prodom
    D.
    PHDsec