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Discussion :: GATE Biotechnology

  1. A complete restriction digestion of a circular plasmid (5000bp) was carried out with HindIII,BamHIandEcoRI individually. Restriction digestion yielded following fragments. 
    Plasmid + HindIII→ 1200bp and3800bp 
    Plasmid + BamHI→5000bp 
    Plasmid + EcoRI→ 2500bp
    The number of sites for EcoRI,BamHIandHindIIIpresent on this plasmid are
    2. A.
    EcoRI-2, BamHI-1, HindIII-2
    B.
    EcoRI-1, BamHI-1, HindIII-2
    C.
    EcoRI-3, BamHI-2, HindIII-1
    D.
    EcoRI-2, BamHI-2, HindIII-1

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    Answer : Option A

    Explanation :

    -NA-

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